The center promotes and facilitates interdisciplinary scientific research for health and for the environment using mass spectrometry-based technologies.
The center manages the mass-spectrometry instrumentation in such a way as to harmonize its use according to personnel, technologies, and available economic resources.
GC/MS
Agilent 5975C EIMSD with head-space sampler
Agilent 5973 EI/CI MSD with EI/CI ion sources
Thermo Finnigan Mat 95 XP Dioxin
HPLC/MS
Agilent 6410 - triple quadrupole MS
Sciex API 5500 - triple quadrupole MS
Shimadzu LC/MC 8060 - triple quadrupole MS
ThermoScientific - LTQ XL Orbitrap
ThermoScientific - Orbitrap Q Exactive
MALDI TOF
AB Sciex4800 TOF/TOF
MASS SPECTROMETRY IMAGING
AB Sciex 4800 TOF/TOF – vacuum MALDI
Themo Orbitrap Q Exactive - atmospheric pressure MALDI
SunChrom - Sun Collect Matrix Sprayer
Open Source Arduino - based IMatrix Sprayer
EASY-FIA is a data preprocessing free software that has been created for researchers working in the field of untarget metabolomics using Flow Injection Analysis High resolution Mass Spectrometry (FIA-HRMS). EASY-FIA is a standalone MATLAB application that provides the user a GUI to set parameters for the elaboration and to keep track of the ongoing processes; importantly it does not require programming expertise. The application retrieves the data (csv files of samples and the relative blanks) generated by any high-resolution mass spectrometers. EASY-FIA performs blank subtraction, m/z alignment and identification based on the Human Metabolome Database (HMDB).
Software availability
The compressed archive which contains EASY-FIA executable code and instruction manual is protected by a password. Whoever wishes to download the software is requested to send an e-mail to centrospettrometriadimassa@marionegri.it.
DOWNLOAD
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- Bagnati R, Terzaghi E, Passoni A, Davoli E, Fattore E, Maspero A, Palmisano G, Zanardini E, Borin S, Di Guardo A. Identification of Sulfonated and Hydroxy-Sulfonated Polychlorinated Biphenyl (PCB) Metabolites in Soil: New Classes of Intermediate Products of PCB Degradation? Environ. Sci.Technol. 2019, 53 (18), 10601–10611.
- Passoni A, Favagrossa M, Colombo L, Bagnati R, Gobbi M, Diomede L, Birolini G, Di Paolo E, Valenza M, Cattaneo E, Salmona M. Efficacy of Cholesterol Nose-to-Brain Delivery for Brain Targeting in Huntington’s Disease. ACS Chem Neurosci 2020, 11 (3), 367–372.
- Bagnati R, Davoli E. Analytical methods for the detection of illicit drugs in wastewaters and surface waters In: Illicit drugs in the environment: Occurrence, analysis, and fate using mass spectrometry. Wiley, Hoboken 2011
- Teoldi F, Lodi M, Benfenati E, Colombo A, Baderna D. Air quality in the Olona Valley and in vitro human health effects. Sci Total Environ 2017; 579: 1929-1939.
- Colombo A, Bettinetti R, Strona G, Cambria F, Fanelli R, Zubair Z, Galli P. Maldives: Anarchipelago that burns. A first survey of PCDD/Fs and DL-PCBs from human activities. Sci Total Environ 2014 ; 497-498 : 499-50.
- Colombo A, Benfenati E, Bugatti SG, Lodi M, Mariani A, Musmeci L, Rotella G, Senese V Ziemacki G, Fanelli R. PCDD/Fs and PCBs in ambient air in a highly industrialized city in Northern Italy. Chemosphere 2013: 90: 2352-2357.
